Western Blot (WB)
(Western Blot analysis of Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) on different lysates using anti-Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: SH-SY-5Y)
Rabbit PAK1/2/3 Polyclonal Antibody | anti-PAK1/2/3 antibody
PAK1/2/3 (Phospho-S144/S141/S139) Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) on different lysates using anti-Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: SH-SY-5Y)
Western Blot (WB)
(Western Blot analysis of Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) on different lysates using anti-Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: SH-SY-5Y)
Immunocytochemistry (ICC)
(ICC staining Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Phospho- PAK1 (S144) +PAK2 (S141) +PAK3 (S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]
Uniprot Description
PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, STE; STE group; STE20 family; PAKA subfamily
Chromosomal Location of Human Ortholog: Xq23
Cellular Component: cytoplasm; plasma membrane; cytosol; endosome
Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; SH3 domain binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: axon guidance; activation of MAPK activity; MAPKKK cascade; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of actin filament polymerization; axonogenesis; positive regulation of neuron apoptosis; T cell costimulation; synapse organization and biogenesis; ephrin receptor signaling pathway; dendrite development; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway
Disease: Mental Retardation, X-linked 30
Research Articles on PAK1/2/3
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Product Notes
The PAK1/2/3 pak3 (Catalog #AAA3014010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAK1/2/3 (Phospho-S144/S141/S139) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAK1/2/3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PAK1/2/3 pak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK1/2/3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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