Western Blot (WB)
((1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MMP14 Polyclonal Antibody | anti-MMP14 antibody
Goat anti-MMP14 Antibody
Expected from Sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from Sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Western Blot: Approx 60kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9kDa according to NP_004986.1). Recommended concentration: 1-3ug/ml.
HC: Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Western Blot (WB)
((1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(MBS422986 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS422986 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.
Protein type: EC 3.4.24.80; Membrane protein, integral; Motility/polarity/chemotaxis; Protease
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: cytoplasm; cytoplasmic vesicle; focal adhesion; Golgi lumen; integral to plasma membrane; plasma membrane
Molecular Function: metalloendopeptidase activity; protein binding; serine-type endopeptidase activity
Biological Process: cell motility involved in cell locomotion; collagen catabolic process; extracellular matrix disassembly; negative regulation of Notch signaling pathway; positive regulation of B cell differentiation; positive regulation of cell growth; positive regulation of cell migration; protein processing; proteolysis
Disease: Winchester Syndrome
Research Articles on MMP14
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Product Notes
The MMP14 mmp14 (Catalog #AAA422986) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MMP14 Antibody reacts with Tested: Human, Mouse Expected from Sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MMP14 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 60kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9kDa according to NP_004986.1). Recommended concentration: 1-3ug/ml. HC: Paraffin embedded Human Liver. Recommended concentration: 5ug/ml. Researchers should empirically determine the suitability of the MMP14 mmp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ESATPLRFRE VP. It is sometimes possible for the material contained within the vial of "MMP14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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