Rabbit PAK3 Polyclonal Antibody | anti-PAK3 antibody

PAK3 (N148) Polyclonal Antibody

Cat. Num. AAA3004251

• Gene Names: PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PAK3; Polyclonal Antibody; PAK3 (N148) Polyclonal Antibody; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3; OPHN3; PAKbeta; anti-PAK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PAK3 (N148) polyclonal antibody detects endogenous levels of PAK3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 544

• Applicable Applications for anti-PAK3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human PAK3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PAK3 (N148) polyclonal antibody in extracts from hela cells.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PAK3 (N148) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-PAK3 antibody

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

NCBI and Uniprot Product Information

• NCBI GI #: 189491751
• NCBI GeneID: 5063
• NCBI Accession #: NP_001121638.1
• NCBI GenBank Nucleotide #: NP_001121638.1
• UniProt Accession #: O75914
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: serine/threonine-protein kinase PAK 3 isoform a
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 3
• NCBI Official Symbol: PAK3
• NCBI Official Synonym Symbols: ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
• NCBI Protein Information: serine/threonine-protein kinase PAK 3
• UniProt Protein Name: Serine/threonine-protein kinase PAK 3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK3
• UniProt Synonym Gene Names: OPHN3; PAK-3
• UniProt Entry Name: PAK3_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]

Uniprot Description

PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, STE; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm; plasma membrane; cytosol; endosome

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; SH3 domain binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: axon guidance; activation of MAPK activity; MAPKKK cascade; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of actin filament polymerization; axonogenesis; positive regulation of neuron apoptosis; T cell costimulation; synapse organization and biogenesis; ephrin receptor signaling pathway; dendrite development; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway

Disease: Mental Retardation, X-linked 30