Rabbit anti-Human, Mouse USP15 Polyclonal Antibody | anti-USP15 antibody

USP15, NT (Ubiquitin Carboxyl-terminal Hydrolase 15, Ubiquitin Thiolesterase 15, Ubiquitin-specific Processing Protease 15, Deubiquitinating Enzyme 15, Unph-2, Unph4, KIAA0529)

Cat. Num. AAA628497

• Gene Names: USP15; UNPH4; KIAA0529; MGC74854; MGC131982; MGC149838
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: USP15; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase 15; Ubiquitin Thiolesterase 15; Ubiquitin-specific Processing Protease 15; Deubiquitinating Enzyme 15; Unph-2; Unph4; KIAA0529); Anti -USP15; anti-USP15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP15. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-USP15 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human USP15 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-USP15 Pab is used in Western blot to detect USP15 in mouse brain tissue lysate.)

Related Product Information for anti-USP15 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP15 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 14149627
• NCBI GeneID: 9958
• NCBI Accession #: NP_006304.1
• NCBI GenBank Nucleotide #: NM_006313.1
• UniProt Accession #: Q08AL5; Q2NL66; Q9H8G9; Q9Y4E8
• Molecular Weight: 109,357 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 15
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 15
• NCBI Official Symbol: USP15
• NCBI Official Synonym Symbols: UNPH4; KIAA0529; MGC74854; MGC131982; MGC149838
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 15; unph-2; ubiquitin thioesterase 15; deubiquitinating enzyme 15; ubiquitin thiolesterase 15; ubiquitin specific protease 15; ubiquitin-specific-processing protease 15
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP15
• UniProt Entry Name: Q08AL5_HUMAN

NCBI Description

Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).[supplied by OMIM]

Uniprot Description

USP15: Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Ubiquitin-specific protease; Ubiquitin conjugating system; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; transforming growth factor beta receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway