Rabbit anti-Human SPINK1 Polyclonal Antibody | anti-SPINK1 antibody

SPINK1 Polyclonal Antibody

Cat. Num. AAA3007392

• Reactivity: Human
• Applications: Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SPINK1; Polyclonal Antibody; SPINK1 Polyclonal Antibody; ISK1; Pancreatic secretory trypsin inhibitor; PCTT; PSTI; Serine protease inhibitor Kazal type 1; Serine protease inhibitor Kazal-type 1; Spink 3; Spink3; TaTI; Tumor associated trypsin inhibitor; Tumor-associated trypsin inhibitor; anti-SPINK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPINK1 polyclonal antibody detects endogenous levels of SPINK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 79

• Applicable Applications for anti-SPINK1 antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:200
• Immunogen: Recombinant full length Human SPINK1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using SPINK1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-SPINK1 antibody

This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT) [MIM: 167800]. A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP) [MIM: 608189]. TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 124856
• UniProt Accession #: P00995
• Molecular Weight: ~ 8kDa
• NCBI Official Full Name: Serine protease inhibitor Kazal-type 1
• UniProt Protein Name: Pancreatic secretory trypsin inhibitor
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK1
• UniProt Synonym Gene Names: PSTI; TATI
• UniProt Entry Name: ISK1_HUMAN

Uniprot Description

SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction

Disease: Tropical Calcific Pancreatitis; Pancreatitis, Hereditary