Human C-C motif chemokine 5 ELISA Kit | CCL5 elisa kit

Human C-C motif chemokine 5 ELISA Kit

Cat. Num. AAA2886866

• Gene Names: CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• Reactivity: Human
• Synonyms: C-C motif chemokine 5; Human C-C motif chemokine 5 ELISA Kit; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTES; CCL5; D17S136E; CCL5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IMA. No significant cross-reactivity or interference between IMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IMA and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 91

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL5 elisa kit

Intended Uses: This IMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IMA. This ELISA kit for research use only!

Principle of the Assay: IMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IMA antibody and an IMA-HRP conjugate. The assay sample and buffer are incubated together with IMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IMA concentration since IMA from samples and IMA-HRP conjugate compete for the anti-IMA antibody binding site. Since the number of sites is limited, as more sites are occupied by IMA from the sample, fewer sites are left to bind IMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22538814
• NCBI GeneID: 6352
• NCBI Accession #: NP_002976.2
• NCBI GenBank Nucleotide #: NM_002985.2
• UniProt Accession #: P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99
• Molecular Weight: 9,990 Da
• NCBI Official Full Name: C-C motif chemokine 5 isoform 1
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• NCBI Protein Information: C-C motif chemokine 5
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: D17S136E; SCYA5; TCP228
• UniProt Entry Name: CCL5_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: CCR1 chemokine receptor binding; CCR4 chemokine receptor binding; CCR5 chemokine receptor binding; chemoattractant activity; chemokine activity; chemokine receptor antagonist activity; chemokine receptor binding; heparin binding; phosphoinositide phospholipase C activity; phospholipase activator activity; protein binding; protein homodimerization activity; protein kinase activity; protein self-association; receptor signaling protein tyrosine kinase activator activity

Biological Process: aging; calcium ion transport; cell-cell signaling; cellular calcium ion homeostasis; cellular protein complex assembly; chemotaxis; chronic inflammatory response; dendritic cell chemotaxis; dibenzo-p-dioxin metabolic process; eosinophil chemotaxis; exocytosis; G-protein coupled receptor protein signaling pathway; inflammatory response; leukocyte adhesion; lipopolysaccharide-mediated signaling pathway; lymphocyte chemotaxis; macrophage chemotaxis; MAPKKK cascade; monocyte chemotaxis; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of viral genome replication; neutrophil activation; neutrophil chemotaxis; phospholipase D activation; positive chemotaxis; positive regulation of angiogenesis; positive regulation of calcium ion transport; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of cellular biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of fever; positive regulation of GTPase activity; positive regulation of homotypic cell-cell adhesion; positive regulation of inflammatory response; positive regulation of innate immune response; positive regulation of JAK-STAT cascade; positive regulation of neuron differentiation; positive regulation of osteoclast differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; positive regulation of translational initiation; positive regulation of tyrosine phosphorylation of STAT protein; positive regulation of viral genome replication; protein kinase B signaling cascade; protein tetramerization; regulation of chronic inflammatory response; regulation of insulin secretion; regulation of T cell activation; response to activity; response to drug; response to estrogen stimulus; response to glucocorticoid stimulus; response to insulin stimulus; response to salt stress; response to toxin; response to virus