Western Blot (WB)
(Western Blot (WB) analysis of BTF3L4 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)
Rabbit BTF3L4 Polyclonal Antibody | anti-BTF3L4 antibody
BTF3L4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
BTF3L4; Polyclonal Antibody; BTF3L4 Polyclonal Antibody; Transcription factor BTF3 homolog 4; Basic transcription factor 3-like 4; anti-BTF3L4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BTF3L4 polyclonal antibody detects endogenous levels of BTF3L4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
78
Applicable Applications for anti-BTF3L4 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human BTF3L4
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of BTF3L4 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of BTF3L4 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)
Related Product Information for anti-BTF3L4 antibody
The initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements such as the TATA box. In addition to RNA polymerase II (Pol II), the transcription factor class II (TFII) family of proteins are required for initiation of transcription, as the first step in the formation of this initiation complex is the stable binding of TFIID to the TATA box. An additional TFII-related protein, BTF3, does not directly associate with the proximal promoter, but rather forms a stable complex with Pol II and facilitates Pol II assembly into the complex. The BTF3 gene is ubiquitously expressed and encodes two protein isoforms, BTF3a and BTF3b, which are produced from alternative splicing. The BTF3 proteins are identical, except that BTF3b lacks the first 44 amino acids at the N-terminal of BTF3a. As a consequence of this deletion, BTF3b is unable to induce transcription, despite being able to bind Pol II. Additionally, BTF3a and BTF3b associate with the widely expressed protein kinase casein kinase II. Casein kinase II phosphorylates BTF3a, as well as TFIIB, and is required for the efficient transcription of the tRNA and 55 rRNA genes by Pol III. BTF3 belongs to the NAC-beta family, which includes several related proteins, such as BTF3L1, BTF3L2, BTF3L3 and BTF3L4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 17kDa
NCBI Official Full Name
transcription factor BTF3 homolog 4 isoform 3
UniProt Protein Name
Transcription factor BTF3 homolog 4
Protein Family
UniProt Gene Name
BTF3L4
Similar Products
Product Notes
The BTF3L4 btf3l4 (Catalog #AAA3006843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTF3L4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BTF3L4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the BTF3L4 btf3l4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTF3L4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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