Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6005352. This demonstrates that MBS6005352 detected the MST1 protein (arrow).)
Rabbit anti-Human MST1 Polyclonal Antibody | anti-MST1 antibody
MST1, CT (MST1, D3F15S2, DNF15S2, HGFL, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, Macrophage-stimulating protein, Hepatocyte growth factor-like protein alpha chain, Hepatocyte growth factor-like protein beta chain) (Azide free
Gene Names
MST1; MSP; HGFL; NF15S2; D3F15S2; DNF15S2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
MST1; Polyclonal Antibody; CT (MST1; D3F15S2; DNF15S2; HGFL; Hepatocyte growth factor-like protein; Macrophage stimulatory protein; Macrophage-stimulating protein; Hepatocyte growth factor-like protein alpha chain; Hepatocyte growth factor-like protein beta chain) (Azide free; Hepatocyte growth factor-like protein beta chain; anti-MST1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MST1.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MST1 antibody
Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 454-483 in the C-terminal region of human MST1.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6005352. This demonstrates that MBS6005352 detected the MST1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6005352. This demonstrates that MBS6005352 detected the MST1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in 293T/17 whole cell lysate (20ug/lane) using MBS6005352 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 80kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in 293T/17 whole cell lysate (20ug/lane) using MBS6005352 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 80kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue using MBS6005352 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005352 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue using MBS6005352 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005352 for immunohistochemistry.)
Product Categories/Family for anti-MST1 antibody
References
1. Latiano, A., et al. Inflamm. Bowel Dis. 16(7):1108-1117(2010). 2. Morgan, A.R., et al. Hum. Immunol. 71(6):602-609(2010). 3. Qiao, M., et al. Mol. Cell 38(4):512-523(2010). 4. McGovern, D.P., et al. Nat. Genet. 42(4):332-337(2010). 5. Oh, H.J., et al. Curr. Biol. 20(5):416-422(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
711
NCBI Official Full Name
hepatocyte growth factor-like protein
NCBI Official Synonym Full Names
macrophage stimulating 1 (hepatocyte growth factor-like)
NCBI Official Symbol
MST1
NCBI Official Synonym Symbols
MSP; HGFL; NF15S2; D3F15S2; DNF15S2
NCBI Protein Information
hepatocyte growth factor-like protein; macrophage-stimulating protein; hepatocyte growth factor-like protein homolog
UniProt Protein Name
Hepatocyte growth factor-like protein
Protein Family
UniProt Gene Name
MST1
UniProt Synonym Gene Names
D3F15S2; DNF15S2; HGFL; MSP
UniProt Entry Name
HGFL_HUMAN
Uniprot Description
MSP: Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. Belongs to the peptidase S1 family. Plasminogen subfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p21
Molecular Function: serine-type endopeptidase activity
Biological Process: sperm motility; spermatogenesis; proteolysis; embryo implantation
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Product Notes
The MST1 mst1 (Catalog #AAA6322575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MST1, CT (MST1, D3F15S2, DNF15S2, HGFL, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, Macrophage-stimulating protein, Hepatocyte growth factor-like protein alpha chain, Hepatocyte growth factor-like protein beta chain) (Azide free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MST1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MST1 mst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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