Rabbit IPP2 Polyclonal Antibody | anti-IPP2 antibody

IPP2 (Q116) Polyclonal Antibody

Cat. Num. AAA3000171

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >107% (by SDS-PAGE).
• Synonyms: IPP2; Polyclonal Antibody; IPP2 (Q116) Polyclonal Antibody; Protein phosphatase inhibitor 2; IPP-2; PPP1R2; anti-IPP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IPP2(Q116) polyclonal antibody detects endogenous levels of IPP2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >107% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 205

• Applicable Applications for anti-IPP2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:2000
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human IPP2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IPP2 (Q116) pAb at 1:2000 dilutionLane1:EC9706 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))

Related Product Information for anti-IPP2 antibody

Two inhibitors of protein phosphatase 1 (PP1) include the phosphatase inhibitor 1 (IPP-1) and phosphatase inhibitor 2 (IPP-2). IPP-2, also known as I-2, interacts with the catalytic subunit of PP1 to form the heterodimer PP1I. The PP1I complex is present in the cytosol of cells in a broad range of vertebrate and invertebrate species. Although the heterodimer itself is inactive, a reversible phosphorylation of IPP-2 at Thr 72 by glycogen-synthase-kinase (GSK3) initiates activation of the heterodimer complex in vitro. Phosphorylation of IPP-2 by casein kinase-II at Ser 86, Ser 120, and Ser 121 enhances the rate of phosphorylation by GSK3 at Thr 72 and effectively activates the heterodimer complex. Besides moderating PP1 activity, IPP-2 may play a role as a chaperone for the correct folding of PP1.

NCBI and Uniprot Product Information

• NCBI GI #: 5453946
• NCBI Accession #: NP_006232.1
• NCBI GenBank Nucleotide #: NP_006232.1
• UniProt Accession #: P41236
• Molecular Weight: ~ 30kDa
• NCBI Official Full Name: protein phosphatase inhibitor 2 isoform 2
• UniProt Protein Name: Protein phosphatase inhibitor 2
• UniProt Gene Name: PPP1R2
• UniProt Synonym Gene Names: IPP2; IPP-2
• UniProt Entry Name: IPP2_HUMAN

Uniprot Description

PPP1R2: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Inhibitor; Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q29

Molecular Function: protein binding; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: glycogen metabolic process; generation of precursor metabolites and energy; negative regulation of catalytic activity; regulation of signal transduction; regulation of phosphoprotein phosphatase activity