Western Blot (WB)
(Western Blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of E. coli lysate.)
Mouse anti-General His Monoclonal Antibody | anti-His antibody
His-tag (4C2) Monoclonal Antibody
Reactivity
General
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
His; Monoclonal Antibody; His-tag (4C2) Monoclonal Antibody; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag; anti-His antibody
Host
Mouse
Reactivity
General
Clonality
Monoclonal
Specificity
His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6×His epitope tag.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-His antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:5000-10,000
IP: 1:50-1:100
IP: 1:50-1:100
Immunogen
Synthetic peptide His-tag.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of E. coli lysate.)
Western Blot (WB)
(Western Blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of E. coli lysate.)
Immunoprecipitation (IP)
(Immunoprecipitation of HEK293T transfected with pcDNA3. 1-MCM5-V5-His (48h) whole cell lysate using His-tag mAb (Sepharose Bead Conjugate) (lane 2 and lane 3). Lane 1 is 30% input. The Western Blot was probed using His-tag # MBS3000028. “up-arrow” (supernatant) ; “down-arrow (deposition))
Immunoprecipitation (IP)
(Immunoprecipitation of HEK293T transfected with pcDNA3. 1-MCM5-V5-His (48h) whole cell lysate using His-tag mAb (Sepharose Bead Conjugate) (lane 2 and lane 3). Lane 1 is 30% input. The Western Blot was probed using His-tag # MBS3000028. “up-arrow” (supernatant) ; “down-arrow (deposition))
Related Product Information for anti-His antibody
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
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Product Notes
The His (Catalog #AAA3000028) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The His-tag (4C2) Monoclonal Antibody reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's His can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:5000-10,000 IP: 1:50-1:100. Researchers should empirically determine the suitability of the His for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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