Mouse anti-General GFP Monoclonal Antibody | anti-GFP antibody

GFP-tag (3A10) Monoclonal Antibody

Cat. Num. AAA3000202

• Reactivity: General
• Applications: Western Blot, Immunoprecipitation
• Purity: Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and t
• Synonyms: GFP; Monoclonal Antibody; GFP-tag (3A10) Monoclonal Antibody; Green fluorescent protein; anti-GFP antibody

• Host: Mouse
• Reactivity: General
• Clonality: Monoclonal
• Specificity: GFP-tag (3A10) mAb detects over-expressed or recombinant proteins containing the GFP epitope tag.
• Purity/Purification: Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and t
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-GFP antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:5000-1:10,000; IP: 1:100-500
• Immunogen: Synthetic peptide GFP-tag.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GFP-tag (3A10) mAb-HRP at 1:2000/1:5000/1:10000 dilutionLane1:HEK293T whole cell lysateLane2:HEK293T whole cell lysate,transfected with pEGFP-C3-ATG3. Lane3:HEK293T whole cell lysate,transfected with pEGFP-C3-ATG12.)

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T,transfected with pEGFP-C3-ATG3, cell lysates using GFP-tag (3A10) mAb (Sepharose Bead Conjugate) (lane 2) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 3). Lane 1 is 30% input. The Western Blot was probed using GFP-tag (3A10) mAb# MBS3000202. “up-arrow” (supernatant) ; “down-arrow” (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of GFP mAbLane1:HEK293T whole cell lysate. Lane2:HEK293T transfected with pEGFP-C3-ATG3. Lane3:HEK293T transfected with pEGFP-C3-ATG12.)

Related Product Information for anti-GFP antibody

Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.