STX1A / Syntaxin-1A Recombinant Protein | STX1A recombinant protein

Human STX1A / Syntaxin-1A Recombinant Protein

• Gene Names: STX1A; STX1; HPC-1; P35-1; SYN1A
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: STX1A / Syntaxin-1A; Human STX1A / Syntaxin-1A Recombinant Protein; Syntaxin-1A; Neuron-specific antigen HPC-1; STX1; STX1A recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 251

• Applicable Applications for STX1A recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF and LGI3. Interacts with SLC6A4. Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU. Interacts with STXBP1 and DAPK1.
• Entry Function: Plays a role in hormone and neurotransmitter exocytosis (By similarity). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 259906442
• NCBI GeneID: 6804
• NCBI Accession #: NP_001159375.1
• NCBI GenBank Nucleotide #: NP_001159375.1
• UniProt Accession #: Q16623
• NCBI Official Full Name: syntaxin-1A isoform 2
• NCBI Official Synonym Full Names: syntaxin 1A
• NCBI Official Symbol: STX1A
• NCBI Official Synonym Symbols: STX1; HPC-1; P35-1; SYN1A
• NCBI Protein Information: syntaxin-1A
• UniProt Protein Name: Syntaxin-1A
• Protein Family: Syntaxin
• UniProt Gene Name: STX1A
• UniProt Synonym Gene Names: STX1
• UniProt Entry Name: STX1A_HUMAN

NCBI Description

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

STX1A: a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: SNARE complex; synaptic vesicle; neuron projection; synaptic vesicle membrane; extracellular region; plasma membrane; integral to membrane; endomembrane system; actomyosin; cytosol; cell junction; secretory granule

Molecular Function: protein binding, bridging; SNAP receptor activity; protein domain specific binding; myosin binding; protein N-terminus binding; calcium-dependent protein binding; ATP-dependent protein binding; SNARE binding; protein binding; chloride channel inhibitor activity; protein heterodimerization activity; myosin head/neck binding; calcium channel inhibitor activity; glycoprotein binding; kinase binding

Biological Process: response to gravity; neurotransmitter secretion; pathogenesis; positive regulation of neurotransmitter secretion; calcium ion-dependent exocytosis; synaptic vesicle fusion to presynaptic membrane; intracellular protein transport; synaptic transmission; vesicle docking; cellular protein metabolic process; glutamate secretion; energy reserve metabolic process; regulation of insulin secretion; secretion by cell; synaptic vesicle docking during exocytosis; positive regulation of exocytosis