Rat Arginase-1 ELISA Kit | Arg1 elisa kit

Rat Arginase-1 ELISA Kit

Cat. Num. AAA289817

• Reactivity: Rat
• Synonyms: Arginase-1; Rat Arginase-1 ELISA Kit; Liver-type arginase; Type I arginase; Arg1; 3.5.3.1; Arg1 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat Arginase-1
• Sequence Length: 323

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 2.5-160 ng/mL
• Sensitivity: <1.25 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.2%
• Inter-assay Precision: Inter-Assay CV: <=7.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arg1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 166091458
• NCBI GeneID: 29221
• NCBI Accession #: NP_058830.2
• NCBI GenBank Nucleotide #: NM_017134.3
• UniProt Accession #: P07824; Q5BK93
• Molecular Weight: 34,973 Da
• NCBI Official Full Name: arginase-1
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: Arg1
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: Arg1
• UniProt Entry Name: ARGI1_RAT

NCBI Description

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase

Cellular Component: cell soma; cytoplasm; extracellular space; mitochondrial outer membrane; neuron projection; nucleus

Molecular Function: arginase activity; manganese ion binding

Biological Process: aging; arginine catabolic process to ornithine; arginine metabolic process; collagen biosynthetic process; female pregnancy; liver development; lung development; mammary gland involution; maternal process involved in pregnancy; positive regulation of endothelial cell proliferation; response to amine stimulus; response to amino acid stimulus; response to axon injury; response to cadmium ion; response to drug; response to herbicide; response to lipopolysaccharide; response to manganese ion; response to methylmercury; response to peptide hormone stimulus; response to selenium ion; response to steroid hormone stimulus; response to vitamin A; response to vitamin E; response to wounding; response to zinc ion; urea cycle