Pig Aminopeptidase N ELISA Kit | ANPEP elisa kit

Pig Aminopeptidase N ELISA Kit

Cat. Num. AAA2880174

• Gene Names: ANPEP; APN; PEPN
• Reactivity: Pig
• Synonyms: Aminopeptidase N; Pig Aminopeptidase N ELISA Kit; AP-N; pAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; gp130; ANPEP; 3.4.11.2; CD13; ANPEP elisa kit

• Reactivity: Pig
• Specificity: Recombinant and natural Pig Aminopeptidase N,AP-N
• Sequence Length: 963

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.2%
• Inter-assay Precision: Inter-Assay CV: <=7.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANPEP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47523628
• NCBI GeneID: 397520
• NCBI Accession #: NP_999442.1
• NCBI GenBank Nucleotide #: NM_214277.1
• UniProt Accession #: P15145; Q29242
• Molecular Weight: 108,832 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Symbol: ANPEP
• NCBI Official Synonym Symbols: APN; PEPN
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• Protein Family: Aminopeptidase
• UniProt Gene Name: ANPEP
• UniProt Synonym Gene Names: AP-N; pAPN; AP-M
• UniProt Entry Name: AMPN_PIG

Uniprot Description

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis (). It is able to degrade Leu-enkephalin and Met-enkephalin but not cholecystokinin CCK8, neuromedin C (GRP-10), somatostatin-14, substance P and vasoactive intestinal peptide. In case of porcine transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCoV) infections, serves as a receptor for TGEV and PRCoV spike glycoprotein in a species-specific manner.