Typical Testing Data/Standard Curve (for reference only)
Rat Interleukin-1 beta ELISA Kit | Il1b elisa kit
Rat Interleukin-1 beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forIL-1 beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
an inflammatory cytokine [RGD, Feb 2006]
Uniprot Description
IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.
Protein type: Cytokine
Cellular Component: autophagic vacuole; cytoplasmic vesicle; cytosol; extracellular region; extracellular space; lysosome; secretory granule; vesicle
Molecular Function: cytokine activity; interleukin-1 receptor binding; protein domain specific binding
Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; acute inflammatory response; chronic inflammatory response to antigenic stimulus; cytokine and chemokine mediated signaling pathway; elevation of cytosolic calcium ion concentration; fever; germ cell programmed cell death; hyaluronan biosynthetic process; immune response; inflammatory response; interleukin-1 beta production; learning and/or memory; leukocyte migration; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; memory; negative regulation of cell proliferation; negative regulation of glutamate secretion; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; neutrophil chemotaxis; ovulation; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of astrocyte differentiation; positive regulation of cell division; positive regulation of chemokine biosynthetic process; positive regulation of fever; positive regulation of glial cell differentiation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of immature T cell proliferation in the thymus; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitosis; positive regulation of neuron apoptosis; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; response to ATP; response to carbohydrate stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to gamma radiation; response to glucocorticoid stimulus; response to heat; response to hypoxia; response to L-ascorbic acid; response to lipopolysaccharide; response to morphine; response to nutrient; response to organic cyclic substance; response to organic nitrogen; response to ozone; response to peptide hormone stimulus; response to vitamin D; sequestering of triacylglycerol; social behavior; wound healing
Research Articles on Il1b
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Product Notes
The Rat Il1b il1b (Catalog #AAA2886910) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886910 ELISA Kit recognizes Rat Il1b. It is sometimes possible for the material contained within the vial of "Interleukin-1 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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