Mouse Piezo-type mechanosensitive ion channel component 1 ELISA Kit | Piezo1 elisa kit

Mouse Piezo-type mechanosensitive ion channel component 1 ELISA Kit

Cat. Num. AAA288115

• Reactivity: Mouse
• Synonyms: Piezo-type mechanosensitive ion channel component 1; Mouse Piezo-type mechanosensitive ion channel component 1 ELISA Kit; Piezo1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Piezo1. No significant cross-reactivity or interference was observed.
• Sequence Length: 2521

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 40.3 pg/mL
• Intra-Assay CV: <=3.5%
• Inter-Assay CV: <=6.5%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Piezo1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Piezo1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Piezo1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Piezo1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Piezo1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 317411795
• UniProt Accession #: E2JF22
• Molecular Weight: 292,002 Da
• NCBI Official Full Name: Piezo-type mechanosensitive ion channel component 1
• UniProt Protein Name: Piezo-type mechanosensitive ion channel component 1
• Protein Family: Piezo-type mechanosensitive ion channel component
• UniProt Gene Name: Piezo1
• UniProt Synonym Gene Names: Fam38a
• UniProt Entry Name: PIEZ1_MOUSE

Uniprot Description

PIEZO1: Component of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. Belongs to the PIEZO family.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Membrane protein, multi-pass

Cellular Component: cell projection; endoplasmic reticulum; integral to membrane; membrane; plasma membrane

Molecular Function: cation channel activity; mechanically-gated ion channel activity; protein binding

Biological Process: cation transport; detection of mechanical stimulus; ion transport; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of integrin activation; regulation of membrane potential; transport