Mouse Apoptosis-inducing factor 1, mitochondrial ELISA Kit | Aifm1 elisa kit

Mouse Apoptosis-inducing factor 1, mitochondrial ELISA Kit

Cat. Num. AAA288204

• Gene Names: Aifm1; Hq; AIF; Pdcd8; AIFsh2
• Reactivity: Mouse
• Synonyms: Apoptosis-inducing factor 1; mitochondrial; Mouse Apoptosis-inducing factor 1; mitochondrial ELISA Kit; Aifm1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Apoptosis-inducing factor 1, mitochondrial. No significant cross-reactivity or interference was observed.
• Sequence Length: 613

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 40.4 pg/mL
• Intra-Assay CV: <=6.6%
• Inter-Assay CV: <=8.5%
• Recovery: 107%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aifm1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis-inducing factor 1, mitochondrial. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apoptosis-inducing factor 1, mitochondrial and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apoptosis-inducing factor 1, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Apoptosis-inducing factor 1, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 594150372
• NCBI GeneID: 26926
• NCBI Accession #: NP_001277293.1
• NCBI GenBank Nucleotide #: NM_001290364.1
• UniProt Accession #: Q9Z0X1
• Molecular Weight: 66,765 Da
• NCBI Official Full Name: apoptosis-inducing factor 1, mitochondrial isoform 2
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated 1
• NCBI Official Symbol: Aifm1
• NCBI Official Synonym Symbols: Hq; AIF; Pdcd8; AIFsh2
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• UniProt Gene Name: Aifm1
• UniProt Synonym Gene Names: Aif; Pdcd8
• UniProt Entry Name: AIFM1_MOUSE

Uniprot Description

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Apoptosis; EC 1.-.-.-; Oxidoreductase

Cellular Component: cytoplasm; cytosol; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrion; nucleus

Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; (R)-2-hydroxyisocaproate dehydrogenase activity; 2-hydroxytetrahydrofuran dehydrogenase activity; 3-ketoglucose-reductase activity; 5-exo-hydroxycamphor dehydrogenase activity; acetoin dehydrogenase activity; aldo-keto reductase activity; arabinose reductase activity; C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity; D-arabinitol dehydrogenase (NADP+) activity; DNA binding; electron-transferring-flavoprotein dehydrogenase activity; epoxide dehydrogenase activity; FAD binding; gluconate dehydrogenase activity; isocitrate dehydrogenase activity; mevaldate reductase activity; NAD(P)H oxidase activity; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH; phenylcoumaran benzylic ether reductase activity; protein binding; protein dimerization activity; steroid dehydrogenase activity; steroid dehydrogenase activity, acting on the CH-CH group of donors; steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; xylose reductase activity

Biological Process: apoptosis; apoptotic mitochondrial changes; caspase activation; cell redox homeostasis; mitochondrial respiratory chain complex I assembly; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptosis; response to oxidative stress