X-Ray Repair Cross Complementing 6 (XRCC6) Recombinant Protein | XRCC6 recombinant protein

Recombinant X-Ray Repair Cross Complementing 6 (XRCC6)

Cat. Num. AAA2018599

• Gene Names: Xrcc6; Ku70; G22p1; Kup70
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: X-Ray Repair Cross Complementing 6 (XRCC6); Recombinant X-Ray Repair Cross Complementing 6 (XRCC6); XRCC6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RYTA RKNVSPYFVA LVPQEEELDD QNIQVTPAGF QLVFLPYADD KRKVPFTEKV MANPEQIDKM KAIVQKLRFT YRSDSFENPV LQQHFRNLEA LALDMMESEQ VVDLTLPKVE AIKKRLGSLA DEFKELVYPP GYNPEGKIAK RKADNEGSAS KKPKVELSEE ELKDLFAKGT LGKLTVPALR DICKAYGLKSGPKKQELLEA LSR
• Sequence Length: 608

• Applicable Applications for XRCC6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Arg397~Arg603 (Accession # Q6AZ64) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 144445950
• NCBI GeneID: 25019
• NCBI Accession #: NP_620780.2
• NCBI GenBank Nucleotide #: NM_139080.2
• UniProt Accession #: Q6AZ64
• Molecular Weight: 27.3kDa
• NCBI Official Full Name: thyroid autoantigen
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 6
• NCBI Official Symbol: Xrcc6
• NCBI Official Synonym Symbols: Ku70; G22p1; Kup70
• NCBI Protein Information: thyroid autoantigen; Ku70 DNA-binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa)
• UniProt Protein Name: Protein Xrcc6
• Protein Family: X-ray repair cross-complementing protein
• UniProt Gene Name: Xrcc6
• UniProt Entry Name: Q6AZ64_RAT

NCBI Description

DNA targeting component of the DNA-dependent protein kinase complex DNA-PK, which plays a role in DNA double strand break repair [RGD, Feb 2006]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: Helicase; Nuclear receptor co-regulator; EC 4.2.99.-; RNA-binding; EC 3.6.4.-; DNA repair, damage; DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; protein complex; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; double-stranded DNA binding; damaged DNA binding; telomeric DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity

Biological Process: positive regulation of neurogenesis; V(D)J recombination; double-strand break repair; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; brain development; response to ionizing radiation; negative regulation of transcription, DNA-dependent; telomere maintenance; response to DNA damage stimulus; DNA duplex unwinding; double-strand break repair via nonhomologous end joining