Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit | MOGAT2 elisa kit

Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit

Cat. Num. AAA287541

• Gene Names: MOGAT2; hDC5; MGAT2; DGAT2L5; DGAT2L5.
• Reactivity: Human
• Synonyms: 2-acylglycerol O-acyltransferase 2 (MOGAT2); Human 2-acylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit; DGAT2L5; FLJ22644; MGAT2; MGC119183; MGC119184; MGC119185; acyl CoA:monoacylglycerol acyltransferase 2; MOGAT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MOGAT2. No significant cross-reactivity or interference between Human MOGAT2 and analogues was observed.
• Sequence Length: 334

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MOGAT2 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate MOGAT2 in samples. An antibody specific for MOGAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MOGAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOGAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOGAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: MOGAT2 contains a phosphate acyltransferase-like domain and 2 predicted N-linked glycosylation sites. Northern blot analysis revealed expression of 7.2-, 3.0-, and 1.3-kb transcripts in liver, stomach, and small intestine, with lower expression in colon and kidney. A probe specific to the truncated splice variant hybridized mainly to the 1.3-kb transcript in stomach. Immunofluorescence analysis of transiently transfected COS-7 cells showed that MGAT2 displayed a pattern consistent with localization in the endoplasmic reticulum.Substrate specificity assays of MOGAT2 demonstrated that MOGAT2 can catalyze the acylation of rac-1- and sn-2-monooleoylglycerols. MOGAT2 uses a broad range of fatty acyl-CoAs but prefers monoacylglycerols containing unsaturated fatty acids.

NCBI and Uniprot Product Information

• NCBI GI #: 37537527
• NCBI GeneID: 80168
• NCBI Accession #: NP_079374.2
• NCBI GenBank Nucleotide #: NM_025098.3
• UniProt Accession #: Q3SYC2; Q3SYC1; Q6ZQZ2; Q86UH6; Q9H630; A8K7I3
• Molecular Weight: 32,298 Da
• NCBI Official Full Name: 2-acylglycerol O-acyltransferase 2
• NCBI Official Synonym Full Names: monoacylglycerol O-acyltransferase 2
• NCBI Official Symbol: MOGAT2
• NCBI Official Synonym Symbols: hDC5; MGAT2; DGAT2L5; DGAT2L5.
• NCBI Protein Information: 2-acylglycerol O-acyltransferase 2
• UniProt Protein Name: 2-acylglycerol O-acyltransferase 2
• Protein Family: 2-acylglycerol O-acyltransferase
• UniProt Gene Name: MOGAT2
• UniProt Synonym Gene Names: DC5; DGAT2L5; MGAT2; hMGAT2; hDC5

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015]

Uniprot Description

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.