Sheep Target of rapamycin complex 2 subunit MAPKAP1 ELISA Kit | MAPKAP1 elisa kit

Sheep Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit

Cat. Num. AAA281070

• Gene Names: MAPKAP1; SIN1
• Reactivity: Sheep
• Synonyms: Target of rapamycin complex 2 subunit MAPKAP1; Sheep Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit; MAPKAP1 elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep MAPKAP1. No significant cross-reactivity or interference between Sheep MAPKAP1 and analogues was observed.
• Sequence Length: 522

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MAPKAP1 elisa kit

Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.
The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 57526311
• NCBI GeneID: 443294
• NCBI Accession #: NP_001009768.1
• NCBI GenBank Nucleotide #: NM_001009768.1
• UniProt Accession #: Q6QD73
• Molecular Weight: 59,175 Da
• NCBI Official Full Name: target of rapamycin complex 2 subunit MAPKAP1
• NCBI Official Symbol: MAPKAP1
• NCBI Official Synonym Symbols: SIN1
• NCBI Protein Information: target of rapamycin complex 2 subunit MAPKAP1
• UniProt Protein Name: Target of rapamycin complex 2 subunit MAPKAP1
• Protein Family: Target of rapamycin complex
• UniProt Gene Name: MAPKAP1
• UniProt Synonym Gene Names: MIP1; SIN1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1

Uniprot Description

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex ().