Human Probable G-protein coupled receptor 179 ELISA Kit | GPR179 elisa kit

Human Probable G-protein coupled receptor 179 (GPR179) ELISA Kit

Cat. Num. AAA280907

• Gene Names: GPR179; CSNB1E; GPR158L; GPR158L1
• Reactivity: Human
• Synonyms: Probable G-protein coupled receptor 179; Human Probable G-protein coupled receptor 179 (GPR179) ELISA Kit; GPR179 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GPR179. No significant cross-reactivity or interference between Human GPR179 and analogues was observed.
• Sequence Length: 2367

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GPR179 elisa kit

GPR179, Belongs to the G-protein coupled receptor 3 family. G protein-coupled receptors (GPCRs), comprise a large protein family of transmembrane receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses.
The class C G-protein-coupled receptors are a class of G-protein coupled receptors that include the metabotropic glutamate receptors and several additional receptors. Family C GPCRs have a large extracellular N-terminus which binds the orthosteric (endogenous) ligand. The shape of this domain is often likened to a clam. Several allosteric ligands to these receptors have been identified and these bind within the seven transmembrane region.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR179 in samples. An antibody specific for GPR179 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR179 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR179 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR179 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 629266054
• NCBI GeneID: 440435
• NCBI Accession #: NP_001004334.3
• NCBI GenBank Nucleotide #: NM_001004334.3
• UniProt Accession #: Q6PRD1
• Molecular Weight: 257,363 Da
• NCBI Official Full Name: probable G-protein coupled receptor 179
• NCBI Official Synonym Full Names: G protein-coupled receptor 179
• NCBI Official Symbol: GPR179
• NCBI Official Synonym Symbols: CSNB1E; GPR158L; GPR158L1
• NCBI Protein Information: probable G-protein coupled receptor 179
• UniProt Protein Name: Probable G-protein coupled receptor 179
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR179
• UniProt Synonym Gene Names: GPR158L; GPR158L1; GPR158-like

NCBI Description

This gene encodes a member of the glutamate receptor subfamily of G protein-coupled receptors. The encoded protein has an EGF-like calcium binding domain and a seven transmembrane domain in the N-terminal region of the protein. Mutations in this gene are associated with congenital stationary night blindness type 1E. [provided by RefSeq, Apr 2012]

Uniprot Description

GPR179: Orphan receptor, involved in vision. Required for signal transduction through retinal depolarizing bipolar cells. Defects in GPR179 are the cause of congenital stationary night blindness type 1E (CSNB1E). An autosomal recessive, non-progressive retinal disorder characterized by impaired night vision, absence of the electroretinogram (ERG) b- wave, and variable degrees of involvement of other visual functions. Affected individuals have an ERG waveform that lacks the b-wave because of failure to transmit the photoreceptor signal through the retinal depolarizing bipolar cells. Belongs to the G-protein coupled receptor 3 family.

Protein type: GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor signaling pathway; visual perception

Disease: Night Blindness, Congenital Stationary, Type 1e