Porcine Glycophorin A ELISA Kit | GYPA elisa kit
Porcine Glycophorin A ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GP39 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GP39 antibody and an GP39-HRP conjugate. The assay sample and buffer are incubated together with GP39-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GP39 concentration since GP39 from samples and GP39-HRP conjugate compete for the anti-GP39 antibody binding site. Since the number of sites is limited, as more sites are occupied by GP39 from the sample, fewer sites are left to bind GP39-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GP39 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
Uniprot Description
GYPA: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Belongs to the glycophorin A family.
Protein type: Membrane protein, integral; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q31.21
Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: viral receptor activity; identical protein binding; protein homodimerization activity
Biological Process: entry of virus into host cell; cytoskeletal anchoring; regulation of response to osmotic stress
Disease: Malaria, Susceptibility To
