Human Phospholipase A2, Group XIIB (PLA2G12B) ELISA Kit | PLA2G12B elisa kit

Human Phospholipase A2, Group XIIB (PLA2G12B) ELISA Kit

Cat. Num. AAA2707137

• Gene Names: PLA2G12B; GXIIB; FKSG71; PLA2G13; GXIIIsPLA2; sPLA2-GXIIB
• Reactivity: Human
• Synonyms: Phospholipase A2; Group XIIB (PLA2G12B); Human Phospholipase A2; Group XIIB (PLA2G12B) ELISA Kit; PLA2G13; FKSG71; GXIIB; Group XIII; Group XIIB secretory phospholipase A2-like protein; PLA2G12B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group XIIB (PLA2G12B).; No significant cross-reactivity or interference between Phospholipase A2, Group XIIB (PLA2G12B) and analogues was observed.
• Sequence Length: 194

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Group XIIB (PLA2G12B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Group XIIB (PLA2G12B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G12B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group XIIB (PLA2G12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group XIIB (PLA2G12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 85567417
• NCBI GeneID: 84647
• UniProt Accession #: Q9BX93; Q52LB2; Q96Q99; B7ZL23
• Molecular Weight: 21,588 Da
• NCBI Official Full Name: Phospholipase A2, group XIIB
• NCBI Official Synonym Full Names: phospholipase A2 group XIIB
• NCBI Official Symbol: PLA2G12B
• NCBI Official Synonym Symbols: GXIIB; FKSG71; PLA2G13; GXIIIsPLA2; sPLA2-GXIIB
• NCBI Protein Information: group XIIB secretory phospholipase A2-like protein
• UniProt Protein Name: Group XIIB secretory phospholipase A2-like protein
• Protein Family: Group XIIB secretory phospholipase A2-like protein
• UniProt Gene Name: PLA2G12B
• UniProt Synonym Gene Names: PLA2G13; GXIII sPLA2-like; GXIIB

NCBI Description

The protein encoded by this gene belongs to the phospholipase A2 (PLA2) group of enzymes, which function in glycolipid hydrolysis with the release of free fatty acids and lysophospholipids. This family member has altered phospholipid-binding properties and is catalytically inactive. The protein is secreted, and together with microsomal triglyceride transfer protein, it functions to regulate HNF4alpha-induced hepatitis C virus infectivity. The expression of this gene is down-regulated in various tumors, suggesting that it may function as a negative regulator of tumor progression. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

Not known; does not seem to have catalytic activity.