Typical Testing Data/Standard Curve (for reference only)
Human PLA2G12B ELISA Kit | PLA2G12B elisa kit
Human PLA2G12B (Phospholipase A2, Group XII B) ELISA Kit
Gene Names
PLA2G12B; GXIIB; FKSG71; PLA2G13; GXIIIsPLA2
Reactivity
Human
Synonyms
PLA2G12B; Human PLA2G12B (Phospholipase A2; Group XII B) ELISA Kit; PLA2G12B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PLA2G12B. No significant cross-reactivity or interference between Human PLA2G12B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PLA2G12B and all the analogues, therefore, cross reaction may still exist.
Sequence Length
194
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G12B elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLA2G12B in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLA2G12B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PLA2G12B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLA2G12B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PLA2G12B. You can calculate the concentration of Human PLA2G12B in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLA2G12B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PLA2G12B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLA2G12B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PLA2G12B. You can calculate the concentration of Human PLA2G12B in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,588 Da
NCBI Official Full Name
PLA2G12B protein
NCBI Official Synonym Full Names
phospholipase A2, group XIIB
NCBI Official Symbol
PLA2G12B
NCBI Official Synonym Symbols
GXIIB; FKSG71; PLA2G13; GXIIIsPLA2
NCBI Protein Information
group XIIB secretory phospholipase A2-like protein; group XIII secreted phospholipase A2; group XIII secretory phospholipase A2-like protein; phospholipase A2, group XIII; sPLA2-GXIIB
UniProt Protein Name
Group XIIB secretory phospholipase A2-like protein
Protein Family
UniProt Gene Name
PLA2G12B
UniProt Synonym Gene Names
PLA2G13; GXIII sPLA2-like; GXIIB
UniProt Entry Name
PG12B_HUMAN
NCBI Description
Phospholipase A2 (PLA2) enzymes catalyze hydrolysis of glycolipids to release free fatty acids and lysophospholipids. PLA2G12B belongs to the PLA2 family, but it is catalytically inactive due to an amino acid change in its active site and has altered phospholipid-binding properties (Rouault et al., 2003 [PubMed 14516201]).[supplied by OMIM, Mar 2008]
Uniprot Description
PLA2G12B: Not known; does not seem to have catalytic activity. Belongs to the phospholipase A2 family.
Protein type: Secreted; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid
Chromosomal Location of Human Ortholog: 10q22.1
Cellular Component: extracellular region
Molecular Function: phospholipase A2 activity; calcium ion binding
Biological Process: cholesterol homeostasis; lipid catabolic process
Research Articles on PLA2G12B
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Product Notes
The Human PLA2G12B pla2g12b (Catalog #AAA2501832) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2501832 ELISA Kit recognizes Human PLA2G12B. It is sometimes possible for the material contained within the vial of "PLA2G12B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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