Horse Vitamin D Binding Protein (DBP) ELISA Kit | DBP elisa kit

Horse Vitamin D Binding Protein (DBP) ELISA Kit

Cat. Num. AAA2703730

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• Reactivity: Horse
• Synonyms: Vitamin D Binding Protein (DBP); Horse Vitamin D Binding Protein (DBP) ELISA Kit; GC; VDBG; VDBP; Group-Specific Component; Gc-globulin; DBP elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP).; No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.
• Sequence Length: 474

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.84ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DBP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 324021743
• NCBI GeneID: 2638
• NCBI Accession #: NP_001191235.1
• NCBI GenBank Nucleotide #: NM_001204306.1
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; D6RAK8
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 1
• NCBI Official Synonym Full Names: GC, vitamin D binding protein
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Remorin
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP-maf

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytosol; extracellular region; extracellular space; lysosomal lumen

Molecular Function: actin binding; vitamin D binding; vitamin transporter activity

Biological Process: vitamin D metabolic process; vitamin transport

Disease: Graves Disease, Susceptibility To, 1