Human Vitamin D-binding protein ELISA Kit | GC elisa kit

Human Vitamin D-binding protein

Cat. Num. AAA8291392

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• Reactivity: Human
• Synonyms: Vitamin D-binding protein; Human Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; GC elisa kit

• Reactivity: Human
• Specificity: The Human Vitamin D-binding protein allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Vitamin D-binding protein within the range of 93.75 pg/ml - 6000 pg/ml.
• Sequence Length: 474

• Samples: Cell culture supernates, serum, plasma, cell lysates, tissue homogenates
• Sensitivity: < 50 pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for GC elisa kit

Principle of the Assay: The MyBioSource' Human Vitamin D-binding protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Vitamin D-binding protein in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Human Vitamin D-binding protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Vitamin D-binding protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Vitamin D-binding protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Vitamin D-binding protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Vitamin D binding protein (VitD BP), also known as DBP and Gc-globulin, is a 58 kDa glycoprotein that circulates at high concentration in the serum and serves as a carrier protein for vitamin D. The transport of vitamin D by VitD BP is important for the function of a wide variety of tissues. VitD BP binds both the 25(OH) and the hormonally active 1,25(OH)2 forms of vitamin D. VitD BP is primarily expressed in hepatocytes and to a lesser extent in the kidney. It delivers vitamin D into cells by Megalin-mediated endocytosis. A selectively deglycosylated form of VitD BP known as macrophage activating factor (MAF) blocks the angiogenic effects of FGF basic, VEGF, and Angiopoietin 2. VitD BP enhances the chemotaxis of monocytes and neutrophils to the activated complement component C5a or C5a des Arg (a C-terminally processed form of C5a). The chemotactic cofactor property of VitD BP is eliminated by binding to 1,25(OH)2 vitamin D, but it is not altered by binding to 25(OH) vitamin D or actin.

NCBI and Uniprot Product Information

• NCBI GI #: 32483410
• NCBI GeneID: 2638
• NCBI Accession #: NP_000574.2
• NCBI GenBank Nucleotide #: NM_000583.3
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; D6RAK8
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 1
• NCBI Official Synonym Full Names: GC, vitamin D binding protein
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Vitamin D-binding protein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP-maf

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.