Human DBP (Vitamin D Binding Protein) CLIA Kit | DBP clia kit

Human DBP (Vitamin D Binding Protein) CLIA Kit

Cat. Num. AAA2530676

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• Reactivity: Human
• Synonyms: DBP (Vitamin D Binding Protein); Human DBP (Vitamin D Binding Protein) CLIA Kit; DBP clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant DBP. No significant cross-reactivity or interference between DBP and analogues was observed.
• Sequence Length: 493

• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DBP clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of DBP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to DBP. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DBP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of DBP. You can calculate the concentration of DBP in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 324021745
• NCBI GeneID: 2638
• NCBI Accession #: NP_001191236.1
• NCBI GenBank Nucleotide #: NM_001204307.1
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; D6RAK8
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 3
• NCBI Official Synonym Full Names: GC, vitamin D binding protein
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: D site-binding protein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP-maf
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: lysosomal lumen; extracellular space; axon; perinuclear region of cytoplasm; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: steroid metabolic process; response to nutrient levels; lactation; female pregnancy; vitamin transport; response to estradiol stimulus; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1