Siglec-10 Recombinant Protein | Siglecg recombinant protein

Recombinant Mouse Siglec-10 (C-Fc)

Cat. Num. AAA2569354

• Gene Names: Siglecg; Siglec-G; Siglec10; mSiglec-G; 9830164H23; A630096C01Rik
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Siglec-10; Recombinant Mouse Siglec-10 (C-Fc); SIGLEC10; SiglecG; Siglec-G; MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; siglec-like gene 2; Siglec-like protein 2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7; Siglecg recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Met19-Lys543

• Species: Mouse
• Tag: C-Fc
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met19-Lys543 is expressed with a Fc tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for Siglecg recombinant protein

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

NCBI and Uniprot Product Information

• NCBI GI #: 151301230
• NCBI GeneID: 243958
• NCBI Accession #: NP_766488
• NCBI GenBank Nucleotide #: NM_172900.3
• UniProt Accession #: Q80ZE3
• Molecular Weight: 76,884 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 10
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin G
• NCBI Official Symbol: Siglecg
• NCBI Official Synonym Symbols: Siglec-G; Siglec10; mSiglec-G; 9830164H23; A630096C01Rik
• NCBI Protein Information: sialic acid-binding Ig-like lectin 10
• UniProt Protein Name: Protein Siglecg
• UniProt Gene Name: Siglecg

Uniprot Description

Siglec-10: a cell surface protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Five alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7|7 B3

Biological Process: hemopoietic progenitor cell differentiation