Siglec-F Recombinant Protein | Siglec5 recombinant protein

Recombinant Mouse Siglec-F (C-6His)

Cat. Num. AAA2569367

• Gene Names: Siglec5; Siglecf; mSiglec-F
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Siglec-F; Recombinant Mouse Siglec-F (C-6His); SAF2; sialic acid binding Ig like lectin 8; SIGLEC-8; SIGLEC8L; SiglecF; Siglec5 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, 1mM EDTA, pH7.4.
• Sequence: Asp18-Thr437

• Species: Mouse
• Tag: C-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Mouse Sialic Acid-binding Ig-like Lectin 5 is produced by our Mammalian expression system and the target gene encoding Asp18-Thr437 is expressed with a 6His tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for Siglec5 recombinant protein

Siglec 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Mouse Siglec-F cDNA encodes a 569 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. The expression of Siglec-F is restricted to the cells of myelomonocytic lineage. Mouse Siglec-F is likely an ortholog of human Siglec-5. Unlike many human CD33-related Siglecs, which show similar binding to both alpha 2,3- and alpha 2,6-linked sialic acids, mouse Siglec-F preferentially recognize alpha 2,3-linked sialic acid.

NCBI and Uniprot Product Information

• NCBI GI #: 402232744
• NCBI GeneID: 233186
• NCBI Accession #: NP_001257948.1
• NCBI GenBank Nucleotide #: NM_001271019.1
• UniProt Accession #: Q920G3
• Molecular Weight: 61,476 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 5 isoform 2
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 5
• NCBI Official Symbol: Siglec5
• NCBI Official Synonym Symbols: Siglecf; mSiglec-F
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5; siglec-5; siglec-F; sialic acid-binding lectin Siglec-F; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin F
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: Siglec5
• UniProt Synonym Gene Names: Siglecf; Siglec-5; Siglec-F; mSiglec-F
• UniProt Entry Name: SIGL5_MOUSE

Uniprot Description

Function: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Predominantly expressed by immature monocytic/myeloid lineage cells in bone marrow. Also found at lower levels in mature neutrophils and monocytes.

Domain: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Sequence similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.