SDS-Page
Siglec-E Recombinant Protein
Recombinant Mouse Siglec-E (C-Fc)
Purity
>95% as determined by reducing SDS-PAGE
Synonyms
Siglec-E; Recombinant Mouse Siglec-E (C-Fc); SiglecE; Sialic Acid Binding Ig-like Lectin E; Siglec-E recombinant protein
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE
Form/Format
Lyophilized from a 0.2um filtered solution of 20mM Tris-HCl, 150mM NaCl, pH8.5.
Sequence
Gln20-Phe355
Species
Mouse
Tag
C-Fc
Endotoxin
<1.0EU per ug as determined by the LAL method.
Protein Construction
Recombinant Mouse Sialic Acid Binding Ig-like Lectin E is produced by our Mammalian expression system and the target gene encoding Gln20-Phe355 is expressed with a Fc tag at the C-terminus.
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
SDS-Page
SDS-Page
Related Product Information for Siglec-E recombinant protein
Siglecs are sialic acid specific I?type lectins that are characterized by an extracellular domain (ECD) with an N?terminal Ig?like V?set domain followed by varying numbers of Ig?like C2?set domains. Mouse Siglec?E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 ? 85kDa member of the CD33?related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglec?9 is the most likely human ortholog of mouse Siglec?E. Siglec?E is expressed as a heavily N?glycosylated disulfide?linked homodimer and shows binding preference for disialic acids in the alpha 2?8 linkage. Siglec?E is up?regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglec?E signaling negatively regulates the LPS?induced production of TNF? alpha and IL?6 by macrophages. Its up?regulation in macrophages parallels the development of endotoxin tolerance. Siglec?E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL?12 p40 production.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Mol. Mass: 64.4kDa
AP Mol. Mass: 85-95kDa
AP Mol. Mass: 85-95kDa
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Product Notes
The Siglec-E (Catalog #AAA2569375) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Gln20-Phe3 55. It is sometimes possible for the material contained within the vial of "Siglec-E, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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