Siglec-10 Biotinylated Active Protein | SIGLEC10 active protein

Recombinant Human Siglec-10 (C-Fc-Avi) Biotinylated

Cat. Num. AAA2569168

• Gene Names: SIGLEC10; SLG2; PRO940; SIGLEC-10
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Siglec-10 Biotinylated; Recombinant Human Siglec-10 (C-Fc-Avi) Biotinylated; SIGLEC10; MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7; SIGLEC10 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Met17--Thr546

• Species: Human
• Tag: C-Fc-Avi
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met17-Thr546 is expressed with a Fc, Avi tag at the C-terminus.
• Activity: Immobilized Anti-Human Siglec10 mAb at 1ug/ml (100ul/well) can bind Biotinylated Human Siglec-10-Fc-Avi . The ED50 of Biotinylated Human Siglec-10-Fc-Avi is 1.45ng/ml.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Activity

Related Product Information for SIGLEC10 active protein

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Product Categories/Family for SIGLEC10 active protein

Tags & Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 283837876
• NCBI GeneID: 89790
• NCBI Accession #: NP_001164627.1
• NCBI GenBank Nucleotide #: NP_001164627.1
• UniProt Accession #: Q96LC7
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 10 isoform 2
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 10
• NCBI Official Symbol: SIGLEC10
• NCBI Official Synonym Symbols: SLG2; PRO940; SIGLEC-10
• NCBI Protein Information: sialic acid-binding Ig-like lectin 10
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 10
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC10
• UniProt Synonym Gene Names: SLG2; Siglec-10
• UniProt Entry Name: SIG10_HUMAN

NCBI Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]

Uniprot Description

Siglec-10: a cell surface protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Five alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: plasma membrane; integral to membrane; extracellular region

Molecular Function: carbohydrate binding

Biological Process: cell adhesion