Western Blot (WB)
(Western Blot analysis of KB cells with Phospho-Histone H3 (Ser10) Polyclonal Antibody)
Aurora A/B Substrate Antibody Kit
Aurora A/B Substrate Antibody Sampler Kit
Applications
Western Blot
Synonyms
Aurora A/B Substrate; Aurora A/B Substrate Antibody Sampler Kit; Aurora A/B Substrate antibody kit
Applicable Applications for Aurora A/B Substrate antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of KB cells with Phospho-Histone H3 (Ser10) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of KB cells with Phospho-Histone H3 (Ser10) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of 293T cells with Phospho-Histone H3 (Ser28) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 293T cells with Phospho-Histone H3 (Ser28) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-p53 (Ser315) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of 293 cells with Phospho-p53 (Ser315) Polyclonal Antibody at dilution of 1:1000)
Related Product Information for Aurora A/B Substrate antibody kit
Background: Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C. Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression. Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation; research investigators have observed overexpression of these kinases in a variety of human cancers. Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines. Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy- terminal end. When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability. Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment and proper alignment of chromosomes during mitosis. Aurora B also targets Ser7 on CENP-A, which in turn regulates Aurora B activity during cytokinesis. Aurora B phosphorylates both Ser10 and Ser28 on histone H3 in concordance with mitotic chromosome condensation. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Aurora A phosphorylates p53 at Ser315 in a cell cycle-dependent manner leading to MDM2-mediated ubiquitination/degradation of p53. Aurora A phosphorylation of Thr210 on PLK promotes mitotic entry following checkpoint-dependent cell cycle arrest.
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Product Notes
The Aurora A/B Substrate (Catalog #AAA2550115) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Aurora A/B Substrate can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Aurora A/B Substrate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora A/B Substrate, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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