Western Blot (WB)
(The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3), DYKDDDDK-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)
Rabbit anti-Human Aurora-C Polyclonal Antibody | anti-AURKC antibody
Aurora-C Antibody (N-term)
Western Blot (WB)
(The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3), DYKDDDDK-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)
Western Blot (WB)
(The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3), DYKDDDDK-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)
Western Blot (WB)
(Aurora Antibody (C) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Aurora antibody detected the Aurora protein (arrow).)
Western Blot (WB)
(Aurora Antibody (C) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Aurora antibody detected the Aurora protein (arrow).)
Tseng, T.C., et al., DNA Cell Biol. 17(10):823-833 (1998).
Bernard, M., et al., Genomics 53(3):406-409 (1998).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
AurC: a member of the AUR family of kinases. May play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. Localized to centrosome from anaphase to cytokinesis. Two splice-variant isoforms have been described.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; AUR family
Chromosomal Location of Human Ortholog: 19q13.43
Cellular Component: spindle microtubule; cytoplasm; condensed chromosome; spindle; spindle pole centrosome; midbody; spindle midzone; condensed nuclear chromosome, pericentric region
Molecular Function: protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; ATP binding; protein kinase activity
Biological Process: positive regulation of cytokinesis; spindle midzone assembly involved in mitosis; meiotic cell cycle; histone modification; regulation of signal transduction; cytokinesis; attachment of spindle microtubules to kinetochore; protein amino acid phosphorylation
Disease: Spermatogenic Failure 5
Research Articles on AURKC
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Product Notes
The AURKC aurkc (Catalog #AAA9205240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 3-38. The Aurora-C Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aurora-C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the AURKC aurkc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora-C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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