p53 antibody kit

Phospho-p53 Antibody Sampler Kit

Cat. Num. AAA2550108

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Applications: Western Blot
• Synonyms: p53; Phospho-p53 Antibody Sampler Kit; p53 antibody kit

• Sequence Length: 393

• Applicable Applications for p53 antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of COS7 cells with Phospho-p53 (Ser6) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of COS7 cells with Phospho-p53 (Ser9) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of HT-29 cells with Phospho-p53 (Ser15) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-p53 (Thr18) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of COS7 cells with Phospho-p53 (Ser20) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of HT29 cells with Phospho-p53 (Ser33) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of COLO205 cells with Phospho-p53 (Ser37) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-p53 (Ser46) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-p53 (Ser315) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of COS7 cells using p53 Polyclonal Antibody at dilution of 1:2000.)

Related Product Information for p53 antibody kit

Background: The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by CK1delta and CK1epsilon both in vitro and in vivo. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response. Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding. Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response.

NCBI and Uniprot Product Information

• NCBI GI #: 23491729
• NCBI GeneID: 7157
• NCBI Official Full Name: P53
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• Protein Family: p53 and DNA damage-regulated protein

NCBI Description

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]