Testing Data
Aurora A Recombinant Protein | AURA recombinant protein
Aurora A, Active
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Scientific Background: AURORA A belongs to a multigenic family of mitotic serine/threonine kinases which are involved in the control of chromosome segregation. AURORA A is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability (1). AURORA A is expressed and active at the highest level during G2-M phase of the cell cycle. Overexpression of AURORA A has been found to be correlated with the grade of various human solid tumours. Ectopic AURORA A overexpression in any culture cell line leads to polyploidy and centrosome amplification (2).
NCBI and Uniprot Product Information
Uniprot Description
AurA: a member of the AUR family of kinases. A cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines. Regulated by phosphorylation-dependent proteasomal degradation. Localized to mitotic centrosomes and spindle microtubules and is required for centrosome maturation. Loss of Aurora A leads to defective mitotic spindles and gross errors in chromosome segregation. Required for centrosome duplication and chromosome segregation. Overexpression in culture drives transformation and aneuploidy, and negatively regulates p53. Amplified or overexpressed in many tumors or cell lines. Found as a skin tumor susceptibility gene in mouse, and a human SNP in a degradation domain is weakly cancer-associated and undergoes allele-specific amplification. Inhibitors: VX-680, ZM447439, Hesperadin, SNS-595.
Protein type: AUR family; EC 2.7.11.1; Kinase, protein; Oncoprotein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 2 H3|2 94.84 cM
Cellular Component: axon hillock; centrosome; condensed nuclear chromosome, pericentric region; cytosol; germinal vesicle; microtubule cytoskeleton; microtubule organizing center; nucleus; perinuclear region of cytoplasm; pronucleus; spindle; spindle microtubule; spindle midzone; spindle pole centrosome
Molecular Function: histone serine kinase activity; protein binding; protein heterodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; ubiquitin protein ligase binding
Biological Process: anterior/posterior axis specification; centrosome cycle; centrosome localization; meiotic cell cycle; meiotic spindle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; mitotic centrosome separation; mitotic spindle organization and biogenesis; negative regulation of apoptosis; negative regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of cytokinesis; regulation of protein stability; response to wounding; spindle assembly involved in female meiosis I; spindle organization and biogenesis
Research Articles on AURA
Similar Products
Product Notes
The AURA aurka (Catalog #AAA515452) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length. AAA Biotech's Aurora A can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the AURA aurka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
