CD10 / Neprilysin / MME Recombinant Protein | CD10 recombinant protein

Recombinant Rat CD10 / Neprilysin / MME Protein (His tag)

Cat. Num. AAA2547367

• Gene Names: Mme; Nep; SFE; CD10
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD10 / Neprilysin / MME; Recombinant Rat CD10 / Neprilysin / MME Protein (His tag); MME; CD10 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 750

• Application Notes: The recombinant rat MME comprises 719 amino acids and has a predicted molecular mass of 82.5 kDa. The apparent molecular mass of the protein is approximately 93 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD10 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

Description: A DNA sequence encoding the rat MME (P07861) (Tyr52-Trp750) was expressed with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6981210
• NCBI GeneID: 24590
• NCBI Accession #: NP_036740.1
• NCBI GenBank Nucleotide #: NM_012608.2
• UniProt Accession #: P07861
• Molecular Weight: 85,795 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metallo-endopeptidase
• NCBI Official Symbol: Mme
• NCBI Official Synonym Symbols: Nep; SFE; CD10
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• UniProt Gene Name: Mme
• UniProt Synonym Gene Names: NEP; Neutral endopeptidase; SFE

NCBI Description

zinc-containing metallo-enzyme; involved in degradation of cardiac peptides [RGD, Feb 2006]

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.11; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: axon; brush border; cytoplasm; dendrite; focal adhesion; membrane; plasma membrane; synapse; synaptic vesicle

Molecular Function: endopeptidase activity; exopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; peptide binding; zinc ion binding

Biological Process: beta-amyloid metabolic process; creatinine metabolic process; kidney development; peptide metabolic process; proteolysis; sensory perception of pain