CD10 / Neprilysin / MME Recombinant Protein | CD10 recombinant protein

Recombinant Human CD10 / Neprilysin / MME Protein (Fc tag)

Cat. Num. AAA2546078

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD10 / Neprilysin / MME; Recombinant Human CD10 / Neprilysin / MME Protein (Fc tag); MME; CALLA; CD10; DKFZp686O16152; MGC126681; MGC126707; NEP; CD10 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 750

• Application Notes: The recombinant human MME/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 936 amino acids and predicts a molecular mass of 106 kDa. As a result of glycosylation, the rh Fc monomer migrates as approximately 120-130 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD10 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

Description: A DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of human membrane metallo-endopeptidase (NP_000893.2) was fused with the Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893.2
• NCBI GenBank Nucleotide #: NM_000902.3
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 85,514 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metalloendopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T; SCA43
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.11; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: axon; brush border; cytoplasm; dendrite; focal adhesion; plasma membrane; synapse; synaptic vesicle

Molecular Function: endopeptidase activity; exopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; peptide binding; protein binding; zinc ion binding

Biological Process: angiotensin maturation; beta-amyloid metabolic process; creatinine metabolic process; kidney development; peptide metabolic process; proteolysis; sensory perception of pain

Disease: Charcot-marie-tooth Disease, Axonal, Type 2t; Membranous Nephropathy, Susceptibility To; Spinocerebellar Ataxia 43