CD10 Recombinant Protein | MME recombinant protein

CD10 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123420

• Gene Names: Mme; Nep; SFE; CD10
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD10; CD10 Protein; Rat; Recombinant (His Tag); Rat CD10/Neprilysin/MME Protein (His Tag); membrane metallo-endopeptidase; MME recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Tyr52-Trp750

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the rat MME (P07861)(Tyr52-Trp750) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MME recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12Dogan, et al. (2000) CD10 and BCL-6 Expression in Paraffin Sections of Normal Lymphoid Tissue and B-Cell Lymphomas. American Journal of Surgical Pathology. 24(6): 846-52.

NCBI and Uniprot Product Information

• NCBI GI #: 6981210
• NCBI GeneID: 24590
• NCBI Accession #: NP_036740.1
• NCBI GenBank Nucleotide #: NM_012608.2
• UniProt Accession #: P07861
• Molecular Weight: 85,795 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metallo-endopeptidase
• NCBI Official Symbol: Mme
• NCBI Official Synonym Symbols: Nep; SFE; CD10
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: CD109 antigen
• UniProt Gene Name: Mme
• UniProt Synonym Gene Names: NEP; Neutral endopeptidase; SFE

NCBI Description

zinc-containing metallo-enzyme; involved in degradation of cardiac peptides [RGD, Feb 2006]

Uniprot Description

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2966343).