Carboxypeptidase B2 / CPB2 Recombinant Protein | CPB2 recombinant protein

Recombinant Mouse Carboxypeptidase B2 / CPB2 Protein (His tag)

Cat. Num. AAA2546717

• Gene Names: Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Carboxypeptidase B2 / CPB2; Recombinant Mouse Carboxypeptidase B2 / CPB2 Protein (His tag); 1110032P04Rik; 4930405E17Rik; AI255929; CPR; Cpu; MGC107573; TAFI; CPB2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 422

• Application Notes: The secreted recombinant mouse CPB2 (pro form)comprises 412 amino acids and has a calculated molecular mass of 48 kDa. As a result of glycosylation, the apparent molecular mass of rmCPB2 is approximately 60 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Phe 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CPB2 recombinant protein

Background: Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Description: A DNA sequence encoding the extracellular domain of mouse CPB2 (Q9JHH6) (Met 1-Thr 422) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 31982712
• NCBI GeneID: 56373
• NCBI Accession #: NP_062749.2
• NCBI GenBank Nucleotide #: NM_019775.3
• UniProt Accession #: Q9JHH6; Q5EBI3; Q9QZF0
• Molecular Weight: 48,871 Da
• NCBI Official Full Name: carboxypeptidase B2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2 (plasma)
• NCBI Official Symbol: Cpb2
• NCBI Official Synonym Symbols: CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: Cpb2
• UniProt Synonym Gene Names: Tafi; CPR; CPU; TAFI

NCBI Description

This gene encodes carboxypeptidase B, a zinc-dependent metalloprotease that cleaves peptide bonds at the C-terminus of protein substrates. The encoded preproprotein undergoes proteolytic activation to generate a mature, functional enzyme, and secreted into plasma. [provided by RefSeq, Jan 2016]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.20; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14|14 D3

Cellular Component: extracellular space

Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: fibrinolysis; negative regulation of fibrinolysis; proteolysis; response to heat