Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at1:2000 dilution Lane 1: HepG2 whole cell lysate
Lane 2: T47D whole cell lysate Lysates/proteinsat 20 ?g per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 136 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit GLG1 Polyclonal Antibody | anti-GLG1 antibody
GLG1 Antibody (C-term)
IHC-P~~1:50~100
Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteinsat 20 ?g per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 136 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at1:2000 dilution Lane 1: HepG2 whole cell lysate
Lane 2: T47D whole cell lysate Lysates/proteinsat 20 ?g per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 136 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2:HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2:HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: mouse cerebellum lysate Lane 3: MCF-7 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Testing Data (TD)
( All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: mouse cerebellum lysate Lane 3: MCF-7 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Western Blot (WB)
( GLG1 Antibody (C-term) (MBS9206193)western blot analysis in HepG2,T47D cell linelysates (35ug/lane).This demonstrates the GLG1 antibody detected the GLG1 protein (arrow).)
Western Blot (WB)
( GLG1 Antibody (C-term) (MBS9206193)western blot analysis in HepG2,T47D cell linelysates (35ug/lane).This demonstrates the GLG1 antibody detected the GLG1 protein (arrow).)
Western Blot (WB)
( Western blot analysis of GLG1 Antibody(C-term) (MBS9206193) in mouse cerebellum tissue lysates (35ug/lane). GLG1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
( Western blot analysis of GLG1 Antibody(C-term) (MBS9206193) in mouse cerebellum tissue lysates (35ug/lane). GLG1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
( GLG1 Antibody (C-term) (MBS9206193) IHCanalysis in formalin fixed and paraffinembedded skeletal muscle followed byperoxidase conjugation of the secondaryantibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinicalrelevance has not been evaluated)
Immunohistochemistry (IHC)
( GLG1 Antibody (C-term) (MBS9206193) IHCanalysis in formalin fixed and paraffinembedded skeletal muscle followed byperoxidase conjugation of the secondaryantibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinicalrelevance has not been evaluated)
Kibriya, M.G., et al. Breast Cancer Res. Treat. 114(3):463-477(2009)
Antoine, M., et al. Oncol. Rep. 21(2):357-362(2009)
Ahn, J., et al. J. Cell. Sci. 118 (PT 8), 1725-1731 (2005)
Bouwmeester, T., et al. Nat. Cell Biol. 6(2):97-105(2004)
NCBI and Uniprot Product Information
Uniprot Description
GLG1: Binds fibroblast growth factor and E-selectin (cell- adhesion lectin on endothelial cells mediating the binding of neutrophils). 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q22.3
Cellular Component: Golgi membrane; Golgi apparatus; extracellular matrix; membrane; integral to membrane; plasma membrane
Molecular Function: receptor binding
Biological Process: regulation of chondrocyte differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; leukocyte migration
Research Articles on GLG1
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Product Notes
The GLG1 glg1 (Catalog #AAA9206193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1152-1179. The GLG1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Chicken, Hamster, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GLG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB~~1:1000 IHC-P~~1:50~100. Researchers should empirically determine the suitability of the GLG1 glg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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