Western Blot (WB)
(Western blot analysis of CPB2 expression in mouse liver extract (lane 1). CPB2 at 48KD was detected using rabbit anti- CPB2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit CPB2 Polyclonal Antibody | anti-CPB2 antibody
Anti-CPB2 Antibody
Gene Names
Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
Reactivity
MouseNo cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
CPB2; Polyclonal Antibody; Anti-CPB2 Antibody; Carboxypeptidase U; CarboxypeptidaseU; CarboxypeptidaseB2; Carboxypeptidase B2; CP B2; CPB 2; CPU; PCPB; Procarboxypeptidase B; Procarboxypeptidase R; Procarboxypeptidase U; TAFI; Q96IY4; anti-CPB2 antibody
Host
Rabbit
Reactivity
Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
422
Applicable Applications for anti-CPB2 antibody
Western Blot (WB)
Application Notes
Western Blot: 0.1-0.5mug/ml; Tested Species: Mouse
Tested Species:In-house tested species with positive results.
Other applications have not been tested.
Tested Species:In-house tested species with positive results.
Other applications have not been tested.
Immunogen
E Coli-derived mouse CPB2 recombinant protein (Position: K165-D387). Mouse CPB2 shares 86.1% and 96.9% amino acid (aa) sequence identity with human and rat CPB2, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of CPB2 expression in mouse liver extract (lane 1). CPB2 at 48KD was detected using rabbit anti- CPB2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CPB2 expression in mouse liver extract (lane 1). CPB2 at 48KD was detected using rabbit anti- CPB2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Related Product Information for anti-CPB2 antibody
Rabbit IgG polyclonal antibody for Carboxypeptidase B2 (CPB2) detection.
Background: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Background: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
References
1. Boffa MB, Reid TS, Joo E, Nesheim ME, Koschinsky ML (May 1999). "Characterization of the gene encoding human TAFI (thrombin-activable fibrinolysis inhibitor; plasma procarboxypeptidase B)". Biochemistry 38 (20): 6547-58.
2. Eaton DL, Malloy BE, Tsai SP, Henzel W, Drayna D (Dec 1991). "Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma". J Biol Chem 266 (32): 21833-8.
3. Tsai SP, Drayna D (Dec 1992). "The gene encoding human plasma carboxypeptidase B (CPB2) resides on chromosome 13". Genomics14 (2): 549-50.
2. Eaton DL, Malloy BE, Tsai SP, Henzel W, Drayna D (Dec 1991). "Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma". J Biol Chem 266 (32): 21833-8.
3. Tsai SP, Drayna D (Dec 1992). "The gene encoding human plasma carboxypeptidase B (CPB2) resides on chromosome 13". Genomics14 (2): 549-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,871 Da
NCBI Official Full Name
carboxypeptidase B2 preproprotein
NCBI Official Synonym Full Names
carboxypeptidase B2 (plasma)
NCBI Official Symbol
Cpb2
NCBI Official Synonym Symbols
CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
NCBI Protein Information
carboxypeptidase B2
UniProt Protein Name
Carboxypeptidase B2
Protein Family
UniProt Gene Name
Cpb2
UniProt Synonym Gene Names
Tafi; CPR; CPU; TAFI
UniProt Entry Name
CBPB2_MOUSE
NCBI Description
This gene encodes carboxypeptidase B, a zinc-dependent metalloprotease that cleaves peptide bonds at the C-terminus of protein substrates. The encoded preproprotein undergoes proteolytic activation to generate a mature, functional enzyme, and secreted into plasma. [provided by RefSeq, Jan 2016]
Uniprot Description
CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; EC 3.4.17.20; Secreted; Protease
Cellular Component: extracellular space
Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity
Biological Process: fibrinolysis; negative regulation of fibrinolysis; proteolysis; response to heat
Research Articles on CPB2
Similar Products
Product Notes
The CPB2 cpb2 (Catalog #AAA178498) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CPB2 Antibody reacts with Mouse No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's CPB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5mug/ml; Tested Species: Mouse Tested Species:In-house tested species with positive results. Other applications have not been tested. Researchers should empirically determine the suitability of the CPB2 cpb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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