RSPO3 / R-spondin 3 Recombinant Protein | RSPO3 recombinant protein

Recombinant Human RSPO3 / R-spondin 3 Protein (aa 1-146, His tag)

Cat. Num. AAA2545882

• Gene Names: RSPO3; PWTSR; THSD2; CRISTIN1
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: RSPO3 / R-spondin 3; Recombinant Human RSPO3 / R-spondin 3 Protein (aa 1-146; His tag); CRISTIN1; FLJ14440; PWTSR; THSD2; RSPO3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 272

• Application Notes: The recombinant human RSPO3 consists of 136 amino acids and predictes a molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh RSPO3 is approximately 22-27 kDa due to different glycosylation.
• Predicted N Terminal: Gln 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for RSPO3 recombinant protein

Background: R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.

Description: A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 18490982
• NCBI GeneID: 84870
• NCBI Accession #: NP_116173.2
• NCBI GenBank Nucleotide #: NM_032784.4
• UniProt Accession #: Q9BXY4; Q5VTV4; Q96K87; B2RC27
• Molecular Weight: 33,233 Da
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: RSPO3
• NCBI Official Synonym Symbols: PWTSR; THSD2; CRISTIN1
• NCBI Protein Information: R-spondin-3
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: RSPO3
• UniProt Synonym Gene Names: PWTSR; THSD2; hPWTSR; hRspo3

NCBI Description

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

Uniprot Description

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: extracellular region

Molecular Function: receptor binding

Biological Process: blood vessel remodeling; sprouting angiogenesis