Typical Testing Data/Standard Curve (for reference only)
Human Atrophin 1 (ATN1) ELISA Kit | ATN1 elisa kit
Human Atrophin 1 (ATN1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ATN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATN1 antibody and an ATN1-HRP conjugate. The assay sample and buffer are incubated together with ATN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATN1 concentration since ATN1 from samples and ATN1-HRP conjugate compete for the anti-ATN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATN1 from the sample, fewer sites are left to bind ATN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATN1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Dentatorubral pallidoluysian atrophy (DRPLA) is a rare neurodegenerative disorder characterized by cerebellar ataxia, myoclonic epilepsy, choreoathetosis, and dementia. The disorder is related to the expansion from 7-23 copies to 49-75 copies of a trinucleotide repeat (CAG/CAA) within this gene. The encoded protein includes a serine repeat and a region of alternating acidic and basic amino acids, as well as the variable glutamine repeat. Alternative splicing results in two transcripts variants that encode the same protein. [provided by RefSeq, Feb 2010]
Uniprot Description
DRPLA: a protein that interacts with the E3 ubiquitin-protein ligase WWP1 and WWP2. May be involved in the third step of ubiquitin conjugation. Relatively high levels in the brain, ovary, testis and prostate. Lower levels in the liver, thymus and leukocytes. Defects are the cause of the neurodegenerative disorders dentatorubral-pallidoluysian atrophy (DRPLA) and Haw River syndrome (HRS).
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: nucleoplasm; nuclear matrix; perinuclear region of cytoplasm; cytoplasm; cell junction; nucleus
Molecular Function: protein domain specific binding; protein binding; toxin receptor binding; transcription corepressor activity
Biological Process: cell migration; neuron apoptosis; central nervous system development; transcription, DNA-dependent; toxin metabolic process; negative regulation of transcription from RNA polymerase II promoter; maintenance of cell polarity
Disease: Dentatorubral-pallidoluysian Atrophy
Research Articles on ATN1
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Product Notes
The Human ATN1 atn1 (Catalog #AAA7220829) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7220829 ELISA Kit recognizes Human ATN1. It is sometimes possible for the material contained within the vial of "Atrophin 1 (ATN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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