Mouse anti-Mouse, Rat CD284 Monoclonal Antibody | anti-CD284 antibody

CD284 Monoclonal Antibody

Cat. Num. AAA2556247

• Gene Names: Tlr4; Lps; Ly87; Ran/M1; Rasl2-8
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CD284; Monoclonal Antibody; CD284 Monoclonal Antibody; ARMD10; CD284 antigen; Homolog of Drosophila toll; hToll; TLR 4; TLR4; TOLL; Toll like receptor 4; Toll-like receptor 4; anti-CD284 antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 2E12C8
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 1.8 mg/mL (varies by lot)
• Sequence Length: 835

• Applicable Applications for anti-CD284 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:500-1:1000
• Immunogen: Recombinant protein corresponding to Mouse TLR4
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD284 Monoclonal Antibody at dilution of 1:400.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded rat spleen using CD284 Monoclonal Antibody at dilution of 1:400.)

Related Product Information for anti-CD284 antibody

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 cooperates with LY96 and CD14 to mediates the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Product Categories/Family for anti-CD284 antibody

Cancer; Cardiovascular; Immunology; Microbiology

NCBI and Uniprot Product Information

• NCBI GI #: 10946594
• NCBI GeneID: 21898
• NCBI Accession #: NP_067272.1
• NCBI GenBank Nucleotide #: NM_021297.3
• UniProt Accession #: Q9QUK6; Q9D691; Q9QZF5; Q9Z203
• Molecular Weight: 95,519 Da
• NCBI Official Full Name: toll-like receptor 4
• NCBI Official Synonym Full Names: toll-like receptor 4
• NCBI Official Symbol: Tlr4
• NCBI Official Synonym Symbols: Lps; Ly87; Ran/M1; Rasl2-8
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• UniProt Gene Name: Tlr4
• UniProt Synonym Gene Names: Lps

NCBI Description

This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]

Uniprot Description

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:9851930, PubMed:9989976, PubMed:20133493). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:24380872). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().