R-Spondin 3/RSPO3 Active Protein | RSPO3 active protein

Human CellExp R-Spondin 3 / RSPO3 (22-146), human recombinant

Cat. Num. AAA844962

• Gene Names: RSPO3; PWTSR; THSD2; CRISTIN1
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: R-Spondin 3/RSPO3; Human CellExp R-Spondin 3 / RSPO3 (22-146); human recombinant; RSPO3; CRISTIN1; PWTSR; THSD2; RSPO3 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Positions: 22-146
• Sequence Length: 272

• Applicable Applications for RSPO3 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to induce activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.5 - 2.0 ng/ml in the presence of 5 ng/mL rmWnt-3a.
• Results: Measured by its ability to induce activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.The ED50 for this effect is typically 0.5 - 2.0 ng/ml in the presence of 5 ng/mL rmWnt-3a.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for RSPO3 active protein

Background: R-Spondin 3 (RSPO3), also called cysteine-rich and single thrombospondin domain containing-1 (CRISTIN1), Protein with TSP type-1 repeat (PWTSR), is a member of the R-Spondin protein family. R-spondins (RSPO) are a recently discovered secretory protein family with four members in human and mouse. Although all four RSPO proteins activate the canonical Wnt pathway, RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. RSPO-3 is expressed ubiquitously and expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is the activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO3 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. RSPO3 also acts as a ligand for frizzled FZD8 and LRP6 and may negatively regulate the TGF-beta pathway.

Product Categories/Family for RSPO3 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 18490982
• NCBI GeneID: 84870
• NCBI Accession #: NP_116173.2
• NCBI GenBank Nucleotide #: NM_032784.4
• UniProt Accession #: Q9BXY4; Q5VTV4; Q96K87; B2RC27
• Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15.3 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 18 kDa and 24 kDa due to different glycosylation.
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: RSPO3
• NCBI Official Synonym Symbols: PWTSR; THSD2; CRISTIN1
• NCBI Protein Information: R-spondin-3
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: RSPO3
• UniProt Synonym Gene Names: PWTSR; THSD2; hPWTSR; hRspo3
• UniProt Entry Name: RSPO3_HUMAN

NCBI Description

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

Uniprot Description

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; frizzled binding; receptor binding

Biological Process: Wnt receptor signaling pathway through beta-catenin