Rabbit anti-Human SerpinA1  Monoclonal Antibody | anti-SerpinA1  antibody

Rabbit anti Human SerpinA1  Monoclonal Antibody

Cat. Num. AAA2543922

• Gene Names: SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
• Reactivity: Human
• Applications: ELISA
• Synonyms: SerpinA1 ; Monoclonal Antibody; Rabbit anti Human SerpinA1 Monoclonal Antibody; anti-SerpinA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 83

• Applicable Applications for anti-SerpinA1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human SerpinA1. The detection limit for Human SerpinA1 is approximately 0.0049 ng/well.
• Immunogen: Recombinant Human SerpinA1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-SerpinA1  antibody

SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. It association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and a complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP). Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.

NCBI and Uniprot Product Information

• NCBI GI #: 957949978
• NCBI GeneID: 5265
• Molecular Weight: 34,755 Da
• NCBI Official Full Name: serpin peptidase inhibitor clade A member 1 isoform 2, partial
• NCBI Official Synonym Full Names: serpin family A member 1
• NCBI Official Symbol: SERPINA1
• NCBI Official Synonym Symbols: PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
• NCBI Protein Information: alpha-1-antitrypsin
• UniProt Protein Name: Alpha-1-antitrypsin
• UniProt Gene Name: SERPINA1
• UniProt Synonym Gene Names: AAT; PI; SPAAT
• UniProt Entry Name: A1AT_HUMAN

NCBI Description

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.