Rabbit EXOSC3 Polyclonal Antibody | anti-EXOSC3 antibody

EXOSC3 Rabbit Polyclonal

Cat. Num. AAA769135

• Gene Names: EXOSC3; p10; PCH1B; RRP40; Rrp40p; CGI-102; hRrp-40; bA3J10.7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: EXOSC3; Polyclonal Antibody; EXOSC3 Rabbit Polyclonal; anti-EXOSC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 275

• Applicable Applications for anti-EXOSC3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Exosome component 3
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS769135 (EXOSC3 antibody) at dilution of 1:50)

Western Blot (WB)

(PC-3 cells were subjected to SDS PAGE followed by western blot with MBS769135 (EXOSC3 antibody) at dilution of 1:400)

NCBI and Uniprot Product Information

• NCBI GI #: 50511943
• NCBI GeneID: 51010
• NCBI Accession #: NP_057126.2
• NCBI GenBank Nucleotide #: NM_016042.3
• Molecular Weight: 17,248 Da
• NCBI Official Full Name: exosome complex component RRP40 isoform 1
• NCBI Official Synonym Full Names: exosome component 3
• NCBI Official Symbol: EXOSC3
• NCBI Official Synonym Symbols: p10; PCH1B; RRP40; Rrp40p; CGI-102; hRrp-40; bA3J10.7
• NCBI Protein Information: exosome complex component RRP40
• UniProt Protein Name: Exosome complex component RRP40
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC3
• UniProt Synonym Gene Names: RRP40
• UniProt Entry Name: EXOS3_HUMAN

NCBI Description

This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2012]

Uniprot Description

EXOSC3: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5. Defects in EXOSC3 are the cause of pontocerebellar hypoplasia type 1B (PCH1B). A severe autosomal recessive neurologic disorder characterized by a combination of cerebellar and spinal motor neuron degeneration beginning at birth. There is diffuse muscle weakness, progressive microcephaly, global developmental delay, and brainstem involvement. Belongs to the RRP40 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Nucleolus; EC 3.1.13.-; Ribonuclease

Chromosomal Location of Human Ortholog: 9p11

Cellular Component: cytoplasm; cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleolus; nucleoplasm; nucleus

Molecular Function: 3'-5'-exoribonuclease activity; exoribonuclease activity; protein binding; RNA binding

Biological Process: DNA deamination; exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); isotype switching; positive regulation of isotype switching; regulation of mRNA stability; rRNA processing