IL-8/CXCL8 ELISA Pair Set | CXCL8 elisa pair set

IL-8/CXCL8 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118182

• Synonyms: IL-8/CXCL8; IL-8/CXCL8 Matched ELISA Antibody Pair Set; Human; Human IL-8/Interleukin-8/CXCL8 ELISA Pair Set; chemokine (C-X-C motif) ligand 8; GCP-1 Matched ELISA Antibody Pair Set; GCP1 Matched ELISA Antibody Pair Set; IL-8 Matched ELISA Antibody Pair Set; IL8 Matched ELISA Antibody Pair Set; Interleukin-8 Matched ELISA Antibody Pair Set; LECT Matched ELISA Antibody Pair Set; LUCT Matched ELISA Antibody Pair Set; LYNAP Matched ELISA Antibody Pair Set; MDNCF Matched ELISA Antibody Pair Set; MONAP Matched ELISA Antibody Pair Set; NAF Matched ELISA Antibody Pair Set; NAP-1 Matched ELISA Antibody Pair Set; NAP1 Matched ELISA Antibody Pair Set; CXCL8 elisa pair set

• Specificity: Quantitative determination of Human IL-8/CXCL8

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 3.13-200pg/mL
• Sensitivity: 3.13pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human IL-8/Interleukin-8/CXCL8 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL-8/Interleukin-8/CXCL8 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use. ; 3. Standard Each vial contains 20 ng of recombinant Human IL-8/Interleukin-8/CXCL8. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 200 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CXCL8 elisa pair set

Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

This IL-8/CXCL8 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL-8/CXCL8. It contains Human IL-8/CXCL8 capture antibody, Human IL-8/CXCL8 detector antibody (HRP) and a highly purified Yeast-expressed recombinant Human IL-8/CXCL8 protein.

Product Categories/Family for CXCL8 elisa pair set

Neuroinflammation; IL17 signaling pathway

References

Mukaida N. (2003) Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases. Am J Physiol Lung Cell Mol Physiol. 284(4): L566-77.Brat DJ, et al. (2005) The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis. Neuro Oncol. 7(2): 122-33.Bendrik C, et al. (2009) Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo. J Immunol. 182(1): 371-8.