Typical Testing Data/Standard Curve (for reference only)
Human secreted frizzled-related protein 5 ELISA Kit | SFRP5 elisa kit
Human SFRP5 (secreted frizzled-related protein 5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SFRP5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SFRP5and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SFRP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SFRP5.You can calculate the concentration of SFRP5in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SFRP5: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina. Belongs to the secreted frizzled-related protein (sFRP) family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region; integral to membrane
Molecular Function: G-protein coupled receptor activity; Wnt receptor activity; Wnt-protein binding
Biological Process: cell differentiation; convergent extension involved in axis elongation; digestive tract morphogenesis; multicellular organismal development; negative regulation of cell proliferation; negative regulation of JNK activity; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Research Articles on SFRP5
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Product Notes
The Human SFRP5 sfrp5 (Catalog #AAA2533360) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533360 ELISA Kit recognizes Human SFRP5. It is sometimes possible for the material contained within the vial of "secreted frizzled-related protein 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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