Canine Cholesteryl Ester Transfer Protein ELISA Kit | CETP elisa kit

Canine Cholesteryl Ester Transfer Protein ELISA Kit

Cat. Num. AAA742387

• Gene Names: CETP; BPIFF; HDLCQ10
• Reactivity: Canine
• Synonyms: Cholesteryl Ester Transfer Protein; Canine Cholesteryl Ester Transfer Protein ELISA Kit; CETP elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This CETP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CETP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CETP elisa kit

Principle of the Assay: CETP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CETP antibody and an CETP-HRP conjugate. The assay sample and buffer are incubated together with CETP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CETP concentration since CETP from samples and CETP-HRP conjugate compete for the anti-CETP antibody binding site. Since the number of sites is limited, as more sites are occupied by CETP from the sample, fewer sites are left to bind CETP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CETP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CETP elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 169636439
• NCBI GeneID: 1071
• NCBI Accession #: NP_000069.2
• NCBI GenBank Nucleotide #: NM_000078.2
• Molecular Weight: 47,787 Da
• NCBI Official Full Name: cholesteryl ester transfer protein isoform 1
• NCBI Official Synonym Full Names: cholesteryl ester transfer protein, plasma
• NCBI Official Symbol: CETP
• NCBI Official Synonym Symbols: BPIFF; HDLCQ10
• NCBI Protein Information: cholesteryl ester transfer protein; lipid transfer protein I; BPI fold containing family F
• UniProt Protein Name: Cholesteryl ester transfer protein
• Protein Family: Cholesteryl ester transfer protein
• UniProt Gene Name: CETP
• UniProt Entry Name: CETP_HUMAN

NCBI Description

The protein encoded by this gene is found in plasma, where it is involved in the transfer of cholesteryl ester from high density lipoprotein (HDL) to other lipoproteins. Defects in this gene are a cause of hyperalphalipoproteinemia 1 (HALP1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

CETP: Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol. Defects in CETP are the cause of hyperalphalipoproteinemia type 1 (HALP1). Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Vesicle; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: lipid transporter activity; phospholipid transporter activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding; triglyceride binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; cholesterol transport; lipid homeostasis; lipoprotein metabolic process; lipid transport; phosphatidylcholine metabolic process; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid transport; triacylglycerol transport; transmembrane transport; phospholipid homeostasis

Disease: Hyperalphalipoproteinemia 1